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Ozone Applications

1,4-Dioxane removal with ozone A New Formulation Based on Ozonated Sunflower Seed Oil: In Vitro Antibacterial and Safety Evaluation AOP Agri-Food Processing Air Treatment Antibacterial Activity of Ozonized Sunflower Oil, Oleozón, Against Staphylococcus aureus and Staphylococcus epidermidis. Antifungal Activity of Olive Oil and Ozonated Olive Oil Against Candida Spp. and Saprochaete Spp. Aquaculture BTEX Remediation under Challenging Site Conditions Using In-Situ Ozone Injection and Soil Vapor Extraction Technologies: A Case Study BTEX removal with ozone Beef (Red Meat) Processing with Ozone Benzene Body Odors Bottled Water Cannabis Catalytic Ozonation of Gasoline Compounds in Model and Natural Water in the Presence of Perfluorinated Alumina Bonded Phases Clean in Place (CIP) Combined Ozone and Ultrasound for the Removal of 1,4-Dioxane from Drinking Water Cooling Tower Cost Effectiveness of Ozonation and AOPs for Aromatic Compound Removal from Water: A Preliminary Study Create your own Ozonated Oils Dairy Farms Degradation of tert-Butyl Alcohol in Dilute Aqueous Solution by an O3/UV Process Drinking Water Drinking Water Disinfection E.coli O157:H7 Reduction with Ozone Effectiveness of Ozone for Inactivation of Escherichia coli and Bacillus Cereus in Pistachios Efficiency of Ozonation and AOP for Methyl-tert-Butylether (MTBE) Removal in Waterworks Ethylbenzene Evaluation of Ozone AOP for Degradation of 1,4-Dioxane Exploring the Potential of Ozonated Oils in Dental Care Exploring the Potential of Ozonated Oils in Hair Care Fire Restoration Food Odors Force Main Treatment Germicidal Properties of Ozonated Sunflower Oil Grain Treatment Groundwater Remediation Hoof Bath Hydroponic Greenhouses In Vitro Antimicrobial Activity of Ozonated Sunflower Oil against Antibiotic-Resistant Enterococcus faecalis Isolated from Endodontic Infection Influence of Storage Temperature on the Composition and the Antibacterial Activity of Ozonized Sunflower Oil Insect Control in Grains Kinetic Analysis of Ozonation Degree Effect on the Physicochemical Properties of Ozonated Vegetable Oils Laundry Laundry Listeria Inactivation with Ozone MTBE removal with ozone Machine Coolant Tanks Measurement of Peroxidic Species in Ozonized Sunflower Oil Mitigation strategies for Salmonella, E. coli O157:H7, and Antimicrobial Resistance Throughout the Beef Production Chain Mold Removal in Grain Mold/Mildew Odors Municipal Water Treatment Mycotoxin Reduction in Grain Nanobubbles Odor Removal Oxidation of Methyl tert-Butyl Ether (MTBE) and Ethyl tert-Butyl Ether (ETBE) by Ozone and Combined Ozone/Hydrogen Peroxide Oxidize Tannins from Water with Ozone Oxy-Oils Ozonated Oils Ozonated Ice & Fish Storage Ozonated Mineral Oil: Preparation, Characterization and Evaluation of the Microbicidal Activity Ozonated Oils: Nature's Remedy for Soothing Bug Bites Ozonated Olive Oil Ozonated Olive Oil Enhances the Growth of Granulation Tissue in a Mouse Model of Pressure Ulcer Ozonated Olive Oil with a High Peroxide Value for Topical Applications: In-Vitro Cytotoxicity Analysis with L929 Cells Ozonation Degree of Vegetable Oils as the Factor of Their Anti-Inflammatory and Wound-Healing Effectiveness Ozonation of Soluble Organics in Aqueous Solutions Using Microbubbles Ozone Gas and Ozonized Sunflower Oil as Alternative Therapies against Pythium Insidiosum Isolated from Dogs Ozone Inactivation of E.Coli at Various O3 Concentrations and Times Ozone Regulations in Food Processing Ozone Regulations in Organic Food Production Ozone in Air Applications Ozone in Sanitation Ozone in Seafood Processing Ozone use for Post-Harvest Processing of Berries Ozone use for Surface Sanitation on Dairy Farms Pet Odors Physico-chemical Characterization and Antibacterial Activity of Ozonated Pomegranate Seeds Oil Pool & Spa Proinflammatory Event of Ozonized Olive Oil in Mice RES Case Studies Resolution Concerning the Use of Ozone in Food Processing Spectroscopic Characterization of Ozonated Sunflower Oil Stability Studies of Ozonized Sunflower Oil and Enriched Cosmetics with a Dedicated Peroxide Value Determination Study of Ozonated Olive Oil: Monitoring of the Ozone Absorption and Analysis of the Obtained Functional Groups Study of Ozonated Sunflower Oil Using 1H NMR and Microbiological Analysis Surface Sanitation TBA Removal with ozone Teat Wash Tobacco Odors Toluene Treatment of Groundwater Contaminated with 1,4-Dioxane, Tetrahydrofuran, and Chlorinated Volatile Organic Compounds Using Advanced Oxidation Processes Treatment of groundwater contaminated with gasoline components by an ozone/UV process Ultra-Pure Water Utilization of Ozone for the Decontamination of Small Fruits Various Antimicrobial Agent of Ozonized Olive Oil Vertical Farming with Ozone Waste Water Treatment Water Re-use Water Treatment Water Treatment Well Water Treatment Xylene

Listeria Inactivation with Ozone

One of the common causes for food safety recalls and illnesses due to food borne pathogens is due to Listeria monocytogenes, more commonly referred to simply as Listeria.  Ozone is an effective tool in the elimination of Listeria in food processing applications.  Ozone is effective at eliminating all bacteria through the process of Lysis

Listeria inactivation with ozone

Listeria is a common term to refer to a specific strain of bacteria species. There are seven (7) known species of Listeria. The specific species L. monocytenes is the cause of Listeriosis, a serious infection caused by eating food contaminated with this strain of bacteria. This disease can be deadly and will pose a greater risk to those with a weakened immune system. Both L.monocytenes, and Liseteriosis, are commonly known only as Listeria, and Listeria illnesses.

Listeria is found in soils, this can lead to fruit and vegetable contamination. Listeria can also be found in all types of meat products, milk, and eggs. Higher risk foods are any uncooked or undercooked foods, unpasteurized milk, raw vegetables, and some ready-to-eat foods.

Click HERE to learn more about Listeria from the CDC (Center for Disease Control and Prevention).

Ozone can be used for the reduction, or elimination of L. monocytenes on food products. Since achieving GRAS approval for the use of ozone for direct contact with food in 2001 the use of ozone for the elimination of L. monocytenes has increased significantly.

To eliminate Listeria or any other bacteria with ozone successful implementation of ozone is necessary. While every application is different, there are a few fundamentals that will apply in most applications.

 


Implementation of Ozone:

Aqueous Ozone:

The most common method of using ozone for pathogen reduction is by dissolving ozone into water. Aqueous ozone is very stable, safe, and easy to manage. Typically, ozone is dissolved into water using an Ozone Injection System and then sprayed onto the surface requiring disinfection. This surface may be a hard equipment surface, or the surface of a food product.

In 2000, the Journal of Food Science published a paper by Kim & Yousef showing the effect of dissolved ozone in a batch reactor on Listeria monocytenes. Dissolved ozone at 0.4 and 0.8 ppm inactivated 4.6 and 5.7 log CFU/ml within 30 seconds. Additional tests were run at higher dissolved ozone levels. Higher dissolved ozone levels did show faster (immediate) inactivation of Listeria monocytenes.

Dissolved ozone can be sprayed on food and produce using spray bars, or other spraying methods. Conveyers work well to allow the sufficient contact time, and offer full coverage of the aqueous ozone. It is important that all of the produce is contacted by the aqueous ozone to achieve desired antimicrobial intervention. Contact times can be varied by altering conveyer speeds, spray tip design, and spray bar design/quantity. If water is already used in an application to wash produce it is very simple to add ozone to this water and achieve an antimicrobial intervention step without any major changes to the current processes.

Gaseous Ozone:

The use of gaseous ozone for the elimination of pathogens is also viable. There is less research showing the effects of gaseous ozone on bacteria. The application of gaseous ozone is dependent upon the temperature, humidity, contact time, and ozone levels. Research has been conducted to determine that gaseous ozone will reduce and inactivate L. monocytenes; however, more research is necessary to determine the effectiveness of ozone within different variables.

Produce in need of disinfection can be placed in chambers, rooms, or even cargo containers for ozone treatment. A sealed area that can contain the produce and ozone gas while maintaining human safety will work. It is necessary to assure sufficient air movement past each piece of produce. Ozone levels from 1.0 – 100 ppm are used in this application with contact times from 20 minutes to 10 hours.

The use of ozone in either gaseous or aqueous forms is effective at inactivating Listeria and should be considered a viable option in many food processing applications.  However, proper care on the engineering and installation should be taken.  

View the papers below for examples of ozone use.  To learn how ozone can be implemented in your applications please contact our office, we would be glad to help.

Ozone Regulations in Food Processing

Ozone regulations in Organic food production

Resolution Concerning the Use of Ozone in Food Processing from original EPRI documents

Original EPRI Direct Food Additive Petition used to gain GRAS approval for ozone

EPRI Global Ozone Handbook

Ozone used for Ecoli O157:H7 Inactivation

 


Efficacy of Ozone in Killing Listeria Monocytogenes on Alfalfa Seeds and Sprouts and Effect on Sensory Quality of Sprouts

Authors: W. N. Wade; A. J. Scouten; K. H. McWatters; R. L. Wick; A.
Demirci; W. F. Fett; and L. R. Beuchata

Abstract

Center for Food Safety, University of Georgia, 1109 Experiment Street, Griffin, Georgia 30223-1797[PARA]  Department of Food Science and Technology,  University of Georgia, 1109 Experiment Street, Griffin, Georgia,  30223-1797[PARA],  Department of Microbiology, 639 Pleasant Street, Morrill,  Science Center IV-N203, University of Massachusetts, Amherst, Massachusetts,  01003-9298[PARA],  Department of Agricultural and Biological Engineering, Life,  Sciences Consortium, Pennsylvania State University, University Park,  Pennsylvania 16802[PARA]eU.S. Department of Agriculture, Agricultural,  Research Service, Eastern Regional Research Center, Food Intervention and,  Technology Research Unit, 600 East Mermaid Lane, Wyndmoor, Pennsylvania,  19038, USA

A study was done to determine the efficacy of aqueous ozone treatment in killing Listeria monocytogenes on inoculated alfalfa seeds and sprouts. Reductions in populations of naturally occurring aerobic microorganisms on sprouts and changes in the sensory quality of sprouts were also determined. The treatment (10 or 20 min) of seeds in water (4°C) containing an initial concentration of 21.8 ± 0.1 g/ml of ozone failed to cause a significant (P 0.05) reduction in populations of L. monocytogenes. The continuous sparging of seeds with ozonated water (initial ozone concentration of 21.3 ± 0.2 g/ml) for 20 min significantly reduced the population by 1.48 log10 CFU/g. The treatment (2 min) of inoculated alfalfa sprouts with water containing 5.0 ± 0.5, 9.0 ± 0.5, or 23.2 ± 1.6 g/ml of ozone resulted in significant (P 0.05) reductions of 0.78, 0.81, and 0.91 log10 CFU/g, respectively, compared to populations detected on sprouts treated with water. Treatments (2 min) with up to 23.3 ± 1.6 g/ml of ozone did not significantly (P > 0.05) reduce populations of aerobic naturally occurring microorganisms. The continuous sparging of sprouts with ozonated water for 5 to 20 min caused significant reductions in L. monocytogenes and natural microbiota compared to soaking in water (control) but did not enhance the lethality compared to the sprouts not treated with continuous sparging. The treatment of sprouts with Ozonated water (20.0 g/ml) for 5 or 10 min caused a significant deterioration in the sensory quality during subsequent storage at 4°C for 7 to 11 days. Scanning electron microscopy of uninoculated alfalfa seeds and sprouts showed physical damage, fungal and bacterial growth, and biofilm formation that provide evidence of factors contributing to the difficulty of killing microorganisms by treatment with ozone and other sanitizers.

Link for full paper found HERE

 


Inactivation of Escherichia coli O157:H7, Listeria Monocytogenes, and Lactobacillus leichmannii by Combinations of Ozone and Pulsed Electric Field

Authors: Unal RKim JGYousef AE.

Abstract

Pulsed electric field (PEF) and ozone technologies are nonthermal processing methods with potential applications in the food industry. This research was performed to explore the potential synergy between ozone and PEF treatments against selected foodborne bacteria. Cells of Lactobacillus leichmannii ATCC 4797, Escherichia coli O157:H7 ATCC 35150, and Listeria monocytogenes Scott A were suspended in 0.1% NaCl and treated with ozone, PEF, and ozone plus PEE Cells were treated with 0.25 to 1.00 microg of ozone per ml of cell suspension, PEF at 10 to 30 kV/cm, and selected combinations of ozone and PEF. Synergy between ozone and PEF varied with the treatment level and the bacterium treated. L. leichmannii treated with PEF (20 kV/cm) after exposure to 0.75 and 1.00 microg/ml of ozone was inactivated by 7.1 and 7.2 log10 CFU/ml, respectively; however, ozone at 0.75 and 1.00 microg/ml and PEF at 20 kV/cm inactivated 2.2, 3.6, and 1.3 log10 CFU/ml, respectively. Similarly, ozone at 0.5 and 0.75 microg/ml inactivated 0.5 and 1.8 log10 CFU/ml of E. coli, PEF at 15 kV/cm inactivated 1.8 log10 CFU/ml, and ozone at 0.5 and 0.75 microg/ml followed by PEF (15 kV/cm) inactivated 2.9 and 3.6 log10 CFU/ml, respectively. Populations of L. monocytogenes decreased 0.1, 0.5, 3.0, 3.9, and 0.8 log10 CFU/ml when treated with 0.25, 0.5, 0.75, and 1.0 microg/ml of ozone and PEF (15 kV/cm), respectively; however, when the bacterium was treated with 15 kV/cm, after exposure to 0.25, 0.5, and 0.75 microg/ml of ozone, 1.7, 2.0, and 3.9 log10 CFU/ml were killed, respectively. In conclusion, exposure of L. leichmannii, E. coli, and L. monocytogenes to ozone followed by the PEF treatment showed a synergistic bactericidal effect. This synergy was most apparent with mild doses of ozone against L. leichmannii.

Link for full paper found HERE

 


 

Elimination of Listeria Monocytogenes Biofilms by Ozone, Chlorine, and Hydogen Peroxide

Authors: Robbins Justin B.; Fisher Christopher W.; Moltz Andrew G.; Martin Scott E.

Abstract

This study evaluated the efficacy of ozone, chlorine, and hydrogen peroxide to destroy Listeria monocytogenes planktonic cells and biofilms of two test strains, Scott A and 10403S. L. monocytogenes was sensitive to ozone (O3), chlorine, and hydrogen peroxide (H2O2). Planktonic cells of strain Scott A were completely destroyed by exposure to 0.25 ppm O3 (8.29-log reduction, CFU per milliliter). Ozone's destruction of Scott A increased when the concentration was increased, with complete elimination at 4.00 ppm O3 (8.07-log reduction, CFU per chip). A 16-fold increase in sanitizer concentration was required to destroy biofilm cells of L. monocytogenes versus planktonic cells of strain Scott A. Strain 10403S required an ozone concentration of 1.00 ppm to eliminate planktonic cells (8.16-log reduction, CFU per milliliter). Attached cells of the same strain were eliminated at a concentration of 4.00 ppm O3 (7.47-log reduction, CFU per chip). At 100 ppm chlorine at 20°C, the number of planktonic cells ofL. monocytogenes 10403S was reduced by 5.77 log CFU/ml after 5 min of exposure and by 6.49 log CFU/ml after 10 min of exposure. Biofilm cells were reduced by 5.79 log CFU per chip following exposure to 100 ppm chlorine at 20°C for 5 min, with complete elimination (6.27 log CFU per chip) after exposure to 150 ppm at 20°C for 1 min. A 3% H2O2 solution reduced the initial concentration of L. monocytogenes Scott A planktonic cells by 6.0 log CFU/ml after 10 min of exposure at 20°C, and a 3.5% H2O2solution reduced the planktonic population by 5.4 and 8.7 log CFU/ml (complete elimination) after 5 and 10 min of exposure at 20°C, respectively. Exposure of cells grown as biofilms to 5% H2O2 resulted in a 4.14-log CFU per chip reduction after 10 min of exposure at 20°C and in a 5.58-log CFU per chip reduction (complete elimination) after 15 min of exposure.

Link for full paper found HERE

 


 

Effect of Ozone and Ultraviolet Irradiation Treatments on Listeria Monocytogenes Populations in Chill Brines

Author: Govindaraj Dev Kumar

Abstract

The efficacy of ozone and ultraviolet light, used in combination, to inactivate Listeria monocytogenes in fresh (9% NaCl, 91.86% transmittance at 254 nm) and spent chill brines (20.5% NaCl, 0.01% transmittance at 254 nm) was determined. Preliminary studies were conducted to optimize parameters for the ozonation of “fresh” and “spent” brines. These include diffuser design, comparison of kit to standard methods to measure residual ozone, studying the effect of ozone on uridine absorbance and determining presence of residual listericidal activity post ozonation.An ozone diffuser was designed using 3/16 inch PVC tubing for the ozonation of brines. The sparger was designed to facilitate better diffusion and its efficiency was tested. The modified sparger diffused 1.44 ppm of ozone after 30 minutes of ozonation and the solution had an excess of 1 ppm in 10 minutes of ozonating fresh brine solution (200ml). Population levels of L. monocytogenes were determined at various time intervals post-ozonation (0, 10, 20, 60 min) to determine the presence of residual listericidal activity. The population post ozonation (0 minutes) was 5.31 Log CFU/ml and was 5.08 Log CFU/ml after a 60 minute interval. Therefore, residual antimicrobial effect was weak. Accuracy of the Vacu-vial Ozone analysis kit was evaluated by comparing the performance of the kit to the standard indigo colorimetric method for measuring residual ozone. The kit was inaccurate in determining residual ozone levels of spent brines and 1% peptone water. Uridine was evaluated as a UV actinometric tool for brine solutions iii that were ozonated before UV treatment. The absorbance of uridine (A262) decreased after ozonation from 0.1329 to 0.0512 for standard 10 minutes UV exposure duration. Absorbance of uridine was influenced by ozone indicating that the presence of ozone may hamper UV fluence determination accuracy in ozone-treated solutions. Upon completion of diffuser design and ozone/UV analysis studies, the effect of ozone-UV combination on L. monocytogenes in fresh and spent brines was evaluated. Ozonation, when applied for 5 minutes, caused a 5.29 mean Log reduction while 5 minutes of UV exposure resulted in a 1.09 mean Log reduction of L. monocytogenes cells in fresh brines. Ten minutes of ozonation led to a 7.44 mean Log reduction and 10 minutes of UV radiation caused a 1.95 mean Log reduction of Listeria in fresh brine. Spent brines required 60 minutes of ozonation for a 4.97 mean Log reduction in L. monocytogenes counts, while 45 minutes resulted in a 4.04 mean Log reduction. Ten minutes of UV exposure of the spent brines resulted in 0.30 mean Log reduction in Listeria cells. A combination of 60 minutes ozonation and 10 minute UV exposure resulted in an excess of 5 log reduction in cell counts. Ozonation did not cause a sufficient increase in the transmittance of the spent brine to aid UV penetration but resulted in apparent color change as indicated by change in L*a*b* values. Ozonation for sufficient time had considerable listericidal activity in fresh brines and spent brines and when combined with UV treatment, is effective reducing L. monocytogenes to undetectable levels in fresh brines.

Link for full paper found HERE

 


Effectiveness of Ozone in Inactivating Listeria Monocytogenes from Milk Samples

Authors: Mariyaselvam Sheelamary, Muthusamy Muthukumar

Abstract

Inactivation of Listeria monocytogenes using ozonation was studied in raw milk and various branded milk samples in and around Coimbatore city. Total of 20 milk samples were obtained from super markets and other places. The PALCAM agar was used in the study to enumerate L. monocytogenes from raw milk and various branded milk samples. Results indicate that all the samples are positive prior to the ozonation process. A controlled flow rate 0.5 m/l of oxygen was used to produce 0.2g/h of ozone. The milk samples were ozonated at 0, 5, 10, and 15 minutes. After treatment the samples are inoculated and L. monocytogenes were enumerated by using listeria PALCAM agar. After 15 minutes ozonation L. monocytogenes were completely eliminated from milk samples. Before and after ozonation the samples were analyzed for protein, carbohydrate, and calcium content. After treatment the nutritional values were slightly different in the milk samples.

Full paper found HERE

 


Inactivation Kinetics of Foodborne Spoilage and Pathogenic Bacteria by Ozone

Authors: J.G. Kim and A.E. Yousef

Abstract

Ozone was tested against Pseudomonas fluorescens, Escherichia coli O157:H7, Leuconostoc mesenteroides, and Listeria monocytogenes. When kinetic data from a batch reactor were fitted to a dose-response model, a 2-phased linear relationship was observed. A continuous ozone reactor was developed to ensure a uniform exposure of bacterial cells to ozone and a constant concentration of ozone during the treatment. Survivors plots in the continuous system were linear initially, followed by a concave downward pattern. Exposure of bacteria to ozone at 2.5 ppm for 40 s caused 5 to 6 log decrease in count. Resistance of tested bacteria to ozone followed this descending order: E. coli O157:H7, P.

Link to full paper found HERE

 


Influence of Catalase and Superoxide Dismutase on Ozone Inactivation of Listeria Monocytogenes

Authors: Christopher W. Fisher, Dongha Lee, Beth-Anne Dodge, Kristen M. Hamman, Justin B. Robbins, and Scott E. Martin

Abstract

The effects of ozone at 0.25, 0.40, and 1.00 ppm on Listeria monocytogenes were evaluated in distilled water and phosphate-buffered saline. Differences in sensitivity to ozone were found to exist among the six strains examined. Greater cell death was found following exposure at lower temperatures. Early stationary-phase cells were less sensitive to ozone than mid-exponential- and late stationary-phase cells. Ozonation at 1.00 ppm of cabbage inoculated with L. monocytogenes effectively inactivated all cells after 5 min. The abilities of in vivo catalase and superoxide dismutase to protect the cells from ozone were also examined. Three listerial test strains were inactivated rapidly upon exposure to ozone. Both catalase and superoxide dismutase were found to protect listerial cells from ozone attack, with superoxide dismutase being more important than catalase in this protection.

Link to full paper found HERE