Bench test set-up with ozone example

We recently set-up some equipment for a customer to perform his own bench testing with ozone.  Below is a description of this set-up and the parts used.  Hopefully, this helps you learn more about the possibilities with ozone and how you can set this up yourself.

More info on ozone bench-test set-ups at this link.


Ozone system for bench testing
Diagram of ozone bench-scale equipment for laboratory testing

The above diagram shows the equipment used and basic plumbing of this equipment. This specific set-up was designed to use ozone gas to treat a dry product. The test column in this application would have a dry edible product in it, ozone would be used to provide disinfection of this food product. Using the ozone analyzer the actual level of ozone passing through the column can be verified, also the ozone analyzer could measure ozone leaving the column to determine the level of ozone consumed by the product.

This system uses an air dryer and a larger air pump as gas flows up to 30 LPM will be used. Target ozone levels of 20 – 200 ppm will be used in this gas flow through the product. The high gas flow and tall colum will help “float” the product to achieve great contact with ozone gas and the product being tested.

ozone bench-test
Example of ozone equipment for lab set-up

The images above and below show the actual products used.  These are the same components shown in the diagram above.  As usual, real-life is not as seamless and tidy as a diagram.  This does give a great example of how this can be set-up in your lab and how much space this may consume.

The ozone products used are:

VMD air dryer 

The VMD-30 air dryer is a vacuum driven heat regenerative air dryer that will provide air dried to -40-deg F dew point at a flow up to 30 LPM.  Perfect to ensure the air fed into the ozone generator is dry, and consistent.  This will ensure reliable ozone levels and measurements.

HTU-500 Ozone Generator

The HTU-500 ozone generator is a great small lab-scale ozone generator.  This unit will operate on air or oxygen, and at pressure or vacuum.  Great versatility for lab scale project.

UV-106M Ozone Analyzer

Measures ozone in air using UV absorption technology.  This unit will measure ozone up to 1,000 ppm accurately with a resolution of only 0.01 ppm.  This great tool can be used for this bench-test set-up and later when this system goes to full scale.


A basic diaphragm compressor was used for this application.  We added a flowmeter with a valve to adjust air flow between 0-30 LPM.  It is important the compressor used does not leak air into itself so we can ensure the dry air is pushed through the ozone generator.

Ozone test column

The ozone test column, shown below, is constructed of 2″ clear PVC and CPVC fittings.  This same column can be used for water tests with only minor differences.  The height of this column ensures ozone gas will pass through the product or water placed in the column for a great distance, this ensures better contact or mass transfer of the ozone.  The cap on top can ensure safety and catch any excess ozone to be measured by a UV ozone analyzer.  A stainless steel diffuser is placed in the TEE at the bottom of this column.  This diffuses the ozone gas into the air or water to ensure great mixing.


ozone equipment for lab scale equipment
Ozone treatment column for bench-test
Column manufactured from PVC to test ozone in lab scale applications
PVC column for ozone bench-test applications

Demonstration of the C-30ZX ozone monitor response speed and alarm function

The C-30ZX ozone monitor is a fixed mount device that will measure ozone from 0-0.14 ppm in 0.02 ppm increments.  This monitor features an internal relay and audible alarm.  The internal relay is user settable at any level from 0.02 – 0.14 in 0.02 ppm increments.  This relay can be used to indicate a remote alarm or shut-down the ozone generation source.  The audible alarm will also sound simultaneously when the relay is set-point is triggered.

The video below displays the response time and the audible alarm triggering.

The EcoSensors C-30ZX is a wall-mounted ozone detector with an instant LED readout, audible safety alarm, and relay switch. The range of ozone measured by this meter is 0-.14 PPM. The main purpose of this sensor is for personnel safety, as 0.14 PPM ozone is the maximum exposure limit allowed by OSHA during an 8-hour work shift.

C-30ZX Ozone Monitor

Purchase EcoSensors C-30ZX Ozone Detector here

The C-30ZX has a rapid readout response to changes in ozone levels. Notice how quickly the LED light readout indicates an increase in the ozone level as the sensor moves near the ozone generator. Then, as it moves away, notice how the LED lights almost instantly reflect the lower levels of ozone.

The C-30ZX has an audible alarm that can be switched on. In this case, the alarm is set to sound at .1 ppm. The alarm can be manually switched off – or, it will also stop sounding as soon as the ozone levels are below the alarm set point.

Bump tester for ozone monitors – ozone puffer

The video below shows the basic functions of the Ozone Puffer.  This is a great tool to use to bump test your ozone monitors quickly without taking them out of service or carrying dangerous calibration gasses to the sensor.

Ozone monitors should be bump tested periodically to ensure they have a positive response to ozone gas and will react to ozone as they should.  Bump testing does not take the place of calibration but is done in-between calibrations to ensure your ozone monitor will react to ozone if, or when a dangerous level of ozone is released into the airspace.

The Ozone Puffer is used for field ozone detector bump tests.

O3 Bump Test Puffer

Purchase Ozone Puffer here

The Ozone Puffer generates ozone internally and then delivers that ozone directly to the ozone sensor. The purpose of the test is not to test the detector’s accuracy, but to ensure that the sensor is responding to ozone.

The Ozone Puffer has 4 features: ozone generator button, LED readout, puffer button, and tubing. To activate the Puffer, hold down the ozone generator button until the LED readout appears as a red number 9. Release the generator button and allow the LED readout to blink about 30 times. When the blinking stops, the Puffer is ready. Quickly press the puffer button while holding the tip of the tubing near the ozone sensor inlet. This delivers a large amount of ozone directly to the ozone sensor. Watch the ozone detector’s readout to ensure that the reading quickly goes up. The purpose is to ensure that the ozone detector is responding to ozone.

Tools for ozone monitor calibration and bump-testing

Ozone monitors and sensors should be calibrated every 6-months to 1-year. This calibration can be done by our technicians to a NIST traceable standard. However, if this is something you prefer to perform on-site we have a few tools you can use to perform calibrations on-site, or bump-test your ozone monitor between calibration intervals.

Learn more about ozone monitor calibration on our main website HERE.

Model 306 Calibration Source

The Model 306 Calibration Source is a UV based ozone source that is portable and easy to use.  This device will provide a known quantity of ozone gas in a sample stream of 3 LPM.  Three usable settable ozone ranges are available to choose from for calibration.  This will allow the calibration of most ozone sensors that have a calibration adapter or a flow-cell where gas can flow to or through the sensor for calibration. This device requires no zero air cylinder and can use ambient air for ozone sensor calibrations.  The accuracy of this device is 2% which is superior to any other portable calibrator available on the market.

Calibrate Ozone monitors with the Model 206
306 Calibration Source

A23-14 Ozone Calibrator

The A23-14 Ozone calibrator is used to calibrate your ozone sensors. This device creates a known level of ozone with the factory calibrated ozone generator. Easy to use, and portable this unit can be carried to your ozone sensor.  Accurate to within 5-10% depending upon the application.

A23-14 Ozone Calibration Kit for field calibration of ozone sensors

Ozone Puffer

The ozone puffer is a simple to use tool to bump-test ozone sensors. Portable and battery powered, this is the easiest method to quickly bump-test your sensor between calibration intervals.  Battery powered unit requires no power cord and is completely portable.

Bump testing is a great method to ensure your sensor is responding to ozone when necessary. Bump testing is not a replacement for calibration. However, bump testing can be done between calibrations to ensure proper response of your ozone sensor, and ensure that a sensor failure has not occured. We suggest bump testing your ozone monitor as often as daily in extremely critical applications. Weekly or montly internvals are common for bump testing your sensor between annual calibration periods.

A23-14 Ozone Calibration Kit for field calibration of ozone sensors
Ozone Puffer.

OG-3 Checker

The OG-3 Calibration Checker is primarily used for Eco-Sensors devices. This will create a known ozone level for bump testing or checking calibration of your sensor between calibration intervals.

OG-3 Ozone calibration checker for accurate bump testing ozone monitors
OG-3 Checker 

Ozone Chambers

We can also provide complete ozone chambers to be used for calibration purposes.  We build standard and custom ozone chambers for ozone exposure testing.  These chambers can also be used for calibrating one, or multiple ozone sensors at one time.  Using a calibration chamber any level of ozone desired can be chosen and entered into the control system, the chamber will maintain that ozone level, by placing your ozone sensors inside the chamber you can validate the readings of your ozone sensors and adjust accordingly.

CH-3 Ozone Mobile Chamber
CH-Series Ozone Chamber


Should you have any further questions on ozone monitor calibration please call our office.  We would be glad to help with your specific needs or to meet any specific criteria you have.

Introduction to the C-30ZX Ozone Monitor

The C-30ZX ozone monitor is a fixed mount ozone detector with a control relay and audible alarm designed to measure ozone from 0-0.14 ppm of ozone in 0.02 ppm increments.  This is a great monitor to use for ozone safety applications where the OSHA 8 hour TWA limit is the primary concern.  The video below shows the basic functions and operation of the C-30ZX ozone monitor.

The EcoSensors C-30ZX Ozone Meter is a fixed monitor with an audible safety alarm and a relay switch. This monitor detects ozone in the range of 0-.14 PPM, which is the OSHA safety limit for ozone exposure during an 8-hour work shift.

C-30ZX Ozone Monitor

Purchase EcoSensors C-30ZX Ozone Detector Here

The ozone level is indicated by an LED readout, which at the low level is green, at the mid-ozone level is yellow, and at the top of its range, 0.14 ppm, is red to indicate danger. The audible safety alarm can be switched on or off. The C-30ZX Ozone Meter is powered on by plugging it in. The only way to power off this detector is to unplug it. The C-30ZX Ozone Meter is designed to be mounted, plugged in, and powered on continuously. It does not have a battery. The calibration certificate is included in the package. The calibration certificate includes the date of calibration, the data involved in the calibration process, and the serial number of the ozone detector unit. This ozone meter should always be powered on for 24 hours before using it for an accurate ozone detecting. The warm-up phase allows the oxidation to burn off the ozone sensor. Once the oxidation has burned off, the sensor can accurately detect ozone.

The C-30ZX monitor reads ozone from 0 – 0.14 ppm and indicates ozone levels with LED bars that illuminate as the ozone levels. 0.1 ppm is the 8-hour OSHA exposure limit for ozone making the C-30ZX an ideal monitor for measuring low-range ozone levels for safety.

Introduction to the EZ-1Z Handheld Ozone Detector

We created a new video about the EZ-1X handheld ozone detector.  This shows the basic functions and what comes in the box with the EZ-1X ozone detector.

The EcoSensors EZ-1X Ozone Detector is a portable detector used for personal safety in the workplace. It responds instantly to ozone in the air, with an LED readout that goes from zero to 0.14 PPM. 0.14 PPM ozone is the OSHA recommended safety level for an 8-hour shift. The LED lights go from green, to yellow, to red indicating the upper OSHA ozone limit is being reached.

EZ-1X handheld ozone sensor

Purchase the EcoSensor EZ-1X Ozone Safety Detector here

Included in the package for the EZ-1X Ozone detector is the monitor itself, the calibration certificate, and the charging cord. The calibration certificate includes the serial number of the unit, data about the calibration process, and the date of calibration. This document needs to be kept for your records, as the ozone monitor should be recalibrated yearly. The EZ-1X ozone detector should be plugged in and fully charged before being used for a work shift. Once fully charged, the EZ-1X detector has a battery life of 8 hours. The monitor needs to go through an initial warmup phase – plugged in and powered on for at least 24 hours before first use. This allows the oxidation to burn off the ozone sensor, allowing the sensor to read ozone levels accurately. The green LED light will automatically light up when the EZ-1X ozone detector is plugged in, indicating that the meter is powered on while also charging. The switch should be in the “AC Power and Bat Chg” position while this ozone detector is plugged in.

When the EZ-1X is plugged in, and the green light is ON it is actually ON and sensing ozone, and can be left that way indefinitely.

The EZ-1X detector reads ozone from 0 – 0.14 ppm and indicates ozone levels with LED bars that illuminate as the ozone levels. 0.1 ppm is the 8-hour OSHA exposure limit for ozone making the EZ-1X an ideal monitor for measuring low-range ozone levels for safety.

Materials ozone resistance chart

A common question when implementing ozone into a new application is how will ozone affect the materials we currently have in place.  Below is a useful chart showing the resistance of common materials to ozone oxidation.

A = Excellent – no effect from ozone in normal operation

B = Good – some oxidation from ozone, these materials can work with low ozone concentrations or short-term (less than 6-month) usage

C = Fair – major effect from ozone oxidation, these materials should only be used for short-term applications or very low ozone levels

D = Poor – severe oxidation from ozone will be noticed quickly.

ABS plastic B – Good
Acetal (Delrin®) C – Fair
Aluminum A – Excellent
Brass B – Good
Bronze B – Good
Buna N (Nitrile) D – Poor
Carbon Steel C – Fair
Cast iron C – Fair
Ceramic A – Excellent
ChemRaz (FFKM) B – Good
Copper A – Excellent
CPVC A – Excellent
EPDM B – Good
Fluorocarbon (FKM) A – Excellent
Hastelloy-C® A – Excellent
HDPE B – Good
Hypalon® A – Excellent
Hytrel® C – Fair
Kalrez A – Excellent
Kel-F® A – Excellent
LDPE C – Fair
Natural rubber D – Poor
Neoprene C – Fair
NORYL® B – Good
Nylon D – Poor
Polycarbonate A – Excellent
Polyetherether Ketone (PEEK) A – Excellent
Polyethylene B – Good
Polypropylene B – Good
Polyurethane A – Excellent
PTFE A – Excellent
PVC B – Good
PVDF (Kynar®) A – Excellent
Silicone A – Excellent
stainless steel – 304 B – Good
stainless steel – 316 A – Excellent
Titanium A – Excellent
Tygon® B – Good
Viton® A – Excellent

More useful information on ozone resistance can be found HERE

We also perform ozone resistance testing in our ozone chambersContact us for information about this service should you have any materials you have specific questions on.

Ozone Combats Red Tide

The USA’s Florida Southern Gulf Coast has been experiencing a severe red tide algae bloom since November 2017.

This particular event is very strong, resulting in major fish kills and disagreeable air quality on  the beach. Red tide is a type of toxic algae that naturally blooms almost every year in Florida. It often dissipates in a few weeks or a month or so. This particular bloom is very strong and has resulted in a state of emergency being declared for the affected areas. Because the tide causes breathing problems, tourism has been severely affected.

Scientists at the Mote Marine Laboratory, Sarasota, FL are conducting experiments to combat the spread of the red tide and prepare for future outbreaks. They are testing a process that

pulls red-tide-infected water from canals into 25,000 galloon tanks, treats it with ozone and pumps it back into the ocean. The initial experiments were successful in removing the red

tide and its toxins and the water chemistry returned to normal within 24 hours. This experiment was featured on several television news shows including CBS and PBS.  On September 23, 2018, Florida Governor, Rick Scott approved a $2.2 million grant to Mote Marine Laboratory to

expand its ozone treatment systems and to conduct new field testing of a specialized clay focused on quelling red tide. The testing should start within a few months.

Mazzei Injector company celebrates 40 years


Angelo and MAry Mazzei celebrated the 40th anniversary of their company

The founders and employees of Mazzei Injector Company celebrated the organization’s 40th anniversary on April 12, 2018

with a look at the evolution from its humble beginnings in Angelo and Mary Mazzei’s Bakersfield garage to its maturation into a world-renowned leader in fluid processing technology. Today, the company–still headed by Angelo Mazzei, who has been joined by his daughter Celia “Cece” Cobar as head of research and development–employs more

than 28 people and manufactures venturi injectors and other specialized fluid handling equipment in Bakersfield. “I think the single best attribute of Mazzei Injector, which has

helped the company succeed for all these years, is the passion for excellence that Angelo has, and that has permeated throughout the company,” said Geoff Whynot, president of Mazzei Injector Company, at a luncheon marking the anniversary of the company’s establishment.

Venturi Principle

Mazzei, who grew up in the San Joaquin Valley and worked on his uncle’s farm after

graduating  from

Fresno State College, built his first injector to efficiently blend fertilizer with irrigation water from the pressurized supply lines feeding off the California Aqueduct. He tapped into the venturi principle, using a specially designed configuration that directed water to increase velocity through a chamber, creating a vacuum that pulled fertilizer into the system and mixed it with the flowing water. With no moving parts and powered by a minimal differential pressure of the water in the line, the system was highly efficient and almost foolproof. Mazzei received his first patent, and founded the company with Mary, in 1978

Since its start in agriculture 40 years ago, Mazzei injectors have become widely used for fluid processing solutions in a wide  range of industries. Winemakers use Mazzei injectors to improve yeast growth during fermentation of their wines. Utilities use them to more efficiently purify drinking water. Food companies and industrial customers use them to reduce odors in their wastewater. Hot tub makers place small Mazzei injectors in their systems to blend ozone into the water for sanitation. And farmers are still using Mazzei injectors to precisely manage fertilizers and crop protection products in irrigation water, and, increasingly, to aerate the root zone. Other Mazzei inventions have further enhanced the efficiency of gas/liquid mixing, which has long challenged engineers.

“For 40 years, Angelo has built more than a company, he’s built a culture–a culture of innovation, a culture of collaboration, a culture of service,” said Jim Lauria, vice president of sales and marketing.

Mazzei Venturis

New research on ozonated olive oil with a high peroxide value

The International Ozone Association has recently published a paper on the use of ozonated olive oil, specifically as it relates to in-vitro cytotoxicity for topical applications.  In recent years, Ozonated olive oil has become a popular application for ozone, however, very little research is published on this application.  Therefore, we are excited to make you aware of this new research.

Ozonated Olive Oil with a High Peroxide Value for Topical Applications: In-Vitro Cytotoxicity Analysis with L929 Cells

IOA logo

Received 17 Jan 2017,  Accepted 07 Jun 2017, Accepted author version posted online: 13 Jun 2017, Published online: 27 Jul 2017

Previous studies have shown that ozonated vegetable oils have been used topically for healing of cutenous wounds. The aim of this study is to evaluate the dose dependent use of ozonated olive oil with high peroxide value (OZ) on the viability of cells for preventing side effects in topical applications. To the best of our knowledge, there are no reports investigaing the effect of peroxide value of ozonated olive oil associated with its cytotoxic activity on mouse non-neoplastc fibroblast cell lines (L929). Therefore, the present study was carried out by using OZ alone and/or in combination with glycerol and olive oil. In our study OZ was prepared by using pure olive oil. Both olive oil and glycerol are non-toxic and can be mixed with OZ uniformly. The cytotoxic activity of samples against L929 fibroblasts was assessed using the tetrazolium salt 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assay. The peroxide value of synthesized OZ was found to be in the range of 2700–2900 mEq O2/kg oil. The OZ/olive oil group did not show any cell death at all concentrations tested (p > 0.05) however OZ/glycerol group showed statistically significant reductions in viability at higher concentrations (p = 0.004–0.006) compared to the control group. Conclusively, using OZ/olive oil with a peroxide value of 2700–2900 mEq O2/kg oil for short-term incubation was non-cytotoxic to the L929 fibroblast cell line.

Access this entire paper and download HERE

Tech tips on creating your own ozonated olive oil HERE

Want more information on the IOA and how to join?  Click HERE