New research on ozonated olive oil with a high peroxide value

The International Ozone Association has recently published a paper on the use of ozonated olive oil, specifically as it relates to in-vitro cytotoxicity for topical applications.  In recent years, Ozonated olive oil has become a popular application for ozone, however, very little research is published on this application.  Therefore, we are excited to make you aware of this new research.

Ozonated Olive Oil with a High Peroxide Value for Topical Applications: In-Vitro Cytotoxicity Analysis with L929 Cells

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Received 17 Jan 2017,  Accepted 07 Jun 2017, Accepted author version posted online: 13 Jun 2017, Published online: 27 Jul 2017

Previous studies have shown that ozonated vegetable oils have been used topically for healing of cutenous wounds. The aim of this study is to evaluate the dose dependent use of ozonated olive oil with high peroxide value (OZ) on the viability of cells for preventing side effects in topical applications. To the best of our knowledge, there are no reports investigaing the effect of peroxide value of ozonated olive oil associated with its cytotoxic activity on mouse non-neoplastc fibroblast cell lines (L929). Therefore, the present study was carried out by using OZ alone and/or in combination with glycerol and olive oil. In our study OZ was prepared by using pure olive oil. Both olive oil and glycerol are non-toxic and can be mixed with OZ uniformly. The cytotoxic activity of samples against L929 fibroblasts was assessed using the tetrazolium salt 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assay. The peroxide value of synthesized OZ was found to be in the range of 2700–2900 mEq O2/kg oil. The OZ/olive oil group did not show any cell death at all concentrations tested (p > 0.05) however OZ/glycerol group showed statistically significant reductions in viability at higher concentrations (p = 0.004–0.006) compared to the control group. Conclusively, using OZ/olive oil with a peroxide value of 2700–2900 mEq O2/kg oil for short-term incubation was non-cytotoxic to the L929 fibroblast cell line.

Access this entire paper and download HERE

Tech tips on creating your own ozonated olive oil HERE

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One thought on “New research on ozonated olive oil with a high peroxide value

  1. We want you inputs for setting up small scale ozonated oil production 10 lit per day

    Suggest us the right ozone generator

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